Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (?-MEM) as a base medium, to evaluate effects Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating (FSH) on vitro development steroid production isolated porcine preantral follicles. Porcine secondary follicles were cultured NCSU23 ?-MEM media for 4 days. Once was determined be optimal culture supplemented FSH (1.5 ng/mL), AMH (50 ng/mL) two hormones. evaluated by measuring follicular growth, morphology hormone production. There no difference between terms follicle survival growth (P > 0.05). However, at day 2, antrum formation rate tended < 0.074) higher than NCSU23. At culture, estradiol progesterone secretion 0.01), while opposite observed testosterone 0.01). The addition and/or did not affect growth. Nevertheless, induced reduced is more effective pig reduces FSH.